This is an improved version of the Blue Fluorescent Protein (mTag BFP; BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag. The R9-tag is a Cell-Penetrating Peptide giving its associated protein the ability to pass through plasma membranes.
For our study we demonstrated that this improved version of mTag BFP had fluorescence of similar levels to the original protein, and was able to stain both Escherichia coli and Pseudomonas putida cells, presumably through internalisation of the protein.
Previous studies have shown R9 associated proteins to be able to enter a variety of cell types (Chang et al., 2005).
DISCLAIMER: This biobrick carries an illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region - For data and design please see CPP-SYFP2.
Sequence and Features
- 10Illegal EcoRI site found at 741
- 12Illegal EcoRI site found at 741
- 21Illegal EcoRI site found at 741
- 23Illegal EcoRI site found at 741
- 25Illegal EcoRI site found at 741
- 1000COMPATIBLE WITH RFC