Coding

Part:BBa_K2455005

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-27)
Revision as of 19:05, 28 October 2017 by Jon UCPH (Talk | contribs)


CPP-mTag BFP

This is an improved version of the mTag BFP (BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag.

DISCLAIMER: This biobrick carries and illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter
//collections/chromoprotein/uppsala
//function/reporter/fluorescence
Parameters
abs399 nm
colorBlue
directionForward
emit456
excite399
functionCell-penetrating, Fluorescent
proteinmTag BFP
tagC-terminal hexa-histidine