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Regulatory
Part:BBa_K2448016:Design
Designed by: jeremy armetta Group: iGEM17_Evry_Paris-Saclay (2017-10-27)
pPsiTac1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006).
Source
DNA synthesis
References
[1] de Boer et al. The tac promoter: A functional hybrid derived from the trp and lac promotersPNAS (1983) 80, 21-25. [2] http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087