Regulatory

Part:BBa_K2448016:Design

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


pPsiTac1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006).

Source

DNA synthesis

References

[1] de Boer HA, Comstock LJ, Vasser M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25. [2] [http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 RegPrecise database]