Device

Part:BBa_K2360008

Designed by: HU RIRONG   Group: iGEM17_SCUT-China_A   (2017-10-27)
Revision as of 13:44, 27 October 2017 by Registry (Talk | contribs)

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rrnb terminater+MerR+pMerR +SRRz+rrnb terminater

It is a high-efficiency mercury detecting device. This device consist two parts, the lysis module (BBa_K2360000) and mercury detecting module(BBa_K2360003).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the mercury ion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None