Part:BBa_K2360008
rrnb terminater+MerR+pMerR +SRRz+rrnb terminater
It is a high-efficiency mercury detecting device. This device consist two parts, the lysis module (BBa_K2360000) and mercury detecting module(BBa_K2360003).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the mercury ion.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Bio-circuit of BBa_K2360008
Experiment
Lysis gene expression
Our device has an obvious contact to mercury ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of mercury ions to prove its function.
Figure 2. the growth curve of mercury detecting device in different concentration of mercury ions.
As shown in the growth curves of E. coli with cadmium detecting device, the growth rate of E. coli BL21 is decline obviously after adding the concentration of Hg2+ from 10-7M to 10-5M, indicating that this device can detect the mercury ions.
Chromogenic Reaction
Our device has an obvious contact to mercury ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of mercury metals.
Figure 3. the comparison between ONPG and X-gal. a. mercury detecting device’s chromogenic reaction at 50mins.
Specificity
We verified the detecting devices specificity of different metal ions.The result indicated that the mercury detecting device has good specificity for specific metal ions.
Figure 4. The growth of the E. coli with mercury detecting device after adding different metal ions and ddH2O
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