DNA
Part:BBa_K2440034
Designed by: Qijie Xu Group: iGEM17_NUDT_CHINA (2017-10-22)
miR172 target sequence
It is the target sequence of miR172, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR172 in an Ago2 dependent manner, that is, knockdown of miR172 was achieved by transfecting cells with miRNA locker.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
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Categories
Parameters
None |