Part:BBa_K2440034

miR172 target sequence

It is the target sequence of miR172, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR172 in an Ago2 dependent manner, that is, knockdown of miR172 was achieved by transfecting cells with miRNA locker.

Ghr-miR172 is observed in cotton. And it is expressed at high levels in the immature ovules but at relatively low levels in fiber-bearing ovules and fibers. This miRNA maybe important to fine-tune developmental and metabolic pathways during early stages of fiber cell initiation and elongation. This miRNA may serve as a negative regulator for cotton fiber development if its targets play a positive role. Alternatively, the miRNA may positively affect cotton fiber development if its targets are negative regulators.¹

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1
  
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

Reference

1.Genome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (Gossypium hirsutum L.)" Pang M, Woodward AW, Agarwal V, Guan X, Ha M, Ramachandran V, Chen X, Triplett BA, Stelly DM, Chen ZJ Genome Biol. 10:R122(2009).