Coding

Part:BBa_K313002

Designed by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-14)
Revision as of 08:42, 27 October 2017 by Gotsmy (Talk | contribs) (→‎Improvements)

flpe DNA recombinase

Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Improvements

• FLPe + NLS: BBa_K2294000 Sequence and Features BBa_K313002 SequenceAndFeatures

[edit]
Categories
//function/recombination/flp
Parameters
None