Coding

Part:BBa_K2294000

Designed by: Mathias Gotsmy   Group: iGEM17_BOKU-Vienna   (2017-10-22)


FLPe + NLS

This part is an improvement of the original part BBa_K313002 (BBa_K2294000 )

This part is a fusion of
• FLPe, a thermostable version of the S. cerevisiae FRT site specific recombinase FLP and
• NLS, a SV40 nuclear localization sequence.

For more information see https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None