Coding

Part:BBa_K2477009

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)
Revision as of 22:25, 26 October 2017 by Registry (Talk | contribs)

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TLP with 6x His-tag 2x GS linker and HLYA tag

This TLP coding region contains two tags. The first is a 6x His-tag for easy protein isolation. The second is an HlyA tag which will allow the protein to be exported from the cell via the HlyBD complex. The two tags are connected via a 2x GS linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 223
    Illegal AgeI site found at 265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 407


[edit]
Categories
Parameters
None