Coding

Part:BBa_K2477009

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


TLP with 6x His-tag 2x GS linker and HLYA tag

This TLP coding region contains a 6x His-tag for easy followed by a 2x GS linker on the C-terminus. This is not an actual basic part, but rather a part stored in the system so as to be combined properly with BBa_K1166002 in another composite part so as to generate the correct sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 120
    Illegal AgeI site found at 223
    Illegal AgeI site found at 265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 407


[edit]
Categories
Parameters
None