Coding

Part:BBa_K2316001:Design

Designed by: Shaw Kar Ming   Group: iGEM17_NTU_SINGAPORE   (2017-10-23)
Revision as of 04:49, 25 October 2017 by Skmhaha (Talk | contribs) (→‎Design Notes)


∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 804
    Illegal BamHI site found at 1622
    Illegal XhoI site found at 2578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2112
    Illegal BsaI site found at 2774
    Illegal BsaI.rc site found at 1027


Design Notes

Created with Gibson

Source

This part is an improvement of BBa_K2316000

References