Coding
Part:BBa_K2316001:Design
Designed by: Shaw Kar Ming Group: iGEM17_NTU_SINGAPORE (2017-10-23)
∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1966
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2112
Illegal BsaI site found at 2774
Illegal BsaI.rc site found at 1027
Design Notes
Created with Gibson
Source
Addgene