Reporter

Part:BBa_K2276007:Design

Designed by: Tianying Chen   Group: iGEM17_SCU_China   (2017-10-24)
Revision as of 09:53, 24 October 2017 by Registry (Talk | contribs)

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a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS.We designed three, and BBa_K2276006 is one of them.


Source

Part:BBa_I13521

References