Reporter

Part:BBa_K2276007:Design

Designed by: Tianying Chen   Group: iGEM17_SCU_China   (2017-10-24)


a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276007 is one of them.

Fig 1.The design result of the RBS in Part:BBa K2276007.
Fig 2.The prediction of the translation rate of Part:BBa I13521

Source

Part:BBa_I13521

References

1.Borujeni A E, Salis H M. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism[J]. Journal of the American Chemical Society, 2016, 138(22):7016.

2.https://parts.igem.org/wiki/index.php?title=Part:BBa_I13521