Regulatory

Part:BBa_K2244006:Design

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-22)
Revision as of 06:53, 23 October 2017 by Qiucheng (Talk | contribs)

ColE promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The entire promoter and operator sequence for SOS regulation system was taken and used directly in the project.


Source

ColE promoter sequence is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.


References