Regulatory
Part:BBa_K2244006:Design
Designed by: Chen Hong Group: iGEM17_SSTi-SZGD (2017-10-22)
ColE promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The entire promoter and operator sequence for SOS regulation system was taken and used directly in the project.
Source
ColE promoter sequence is derived from the promoter region of colicin E gene located in the ColE1 plasmid of E.coli. ColE promoter contains a ‘SOS’ operator region that allows the binding of LexA protein to repress transcription.