Coding

Part:BBa_K2332315

Designed by: Camillo Moschner   Group: iGEM17_UCL   (2017-10-22)
Revision as of 23:16, 22 October 2017 by CamilloMoschner (Talk | contribs)


Plasma membrane delivery system (PMDS, mammalian)

Plasma Membrane Delivery System (PMDS)
Function Delivery of protein (e.g. mCherry) to the PM
Use in Mammalian cells
Abstraction Hierarchy Part
RFC standard RFC10, RFC23 & RFC25 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:UCL UCL iGEM 2017]

This coding region translates a transmembrane domain which is destined to go to the plasma membrane via the secretory pathway. While doing so it delivers any 'cytosolic domain insert' that has been cloned into this BioBrick in a plug-and-play via the KpnI-BamHI restriction sites.



Usage and Biology

As stated in the original paper:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2485
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704
    Illegal BsaI.rc site found at 1471



Reference

Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods. 14(4):391-394 (2017) NCBI

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Categories
Parameters
None