Part:BBa_K2332020
LuxCDABE (E. coli bioluminescence)
This part encodes the production of the proteins required for bacterial luminescence including luciferase and its subtrates from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2562
Illegal AgeI site found at 2829
Illegal AgeI site found at 3357
Illegal AgeI site found at 3938
Illegal AgeI site found at 4385
Illegal AgeI site found at 5263
Illegal AgeI site found at 5478 - 1000COMPATIBLE WITH RFC[1000]
| None |