Coding

Part:BBa_K2332020

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-18)


LuxCDABE (E. coli bioluminescence)

This part encodes the production of the proteins required for bacterial luminescence including luciferase and its substrates from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
    Illegal AgeI site found at 2829
    Illegal AgeI site found at 3357
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 5263
    Illegal AgeI site found at 5478
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None