Coding

Part:BBa_K2294000

Designed by: Mathias Gotsmy   Group: iGEM17_BOKU-Vienna   (2017-10-22)
Revision as of 14:00, 22 October 2017 by Registry (Talk | contribs)

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FLPe + NLS

This part is a fusion between the SV40 nuclear localization signal and a thermostable version of the Saccharomyces cerevisiae site-specific recombinase FLP. Recombination occurs at FRT sequences.See https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None