Coding

Part:BBa_K2332020

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-18)
Revision as of 22:57, 18 October 2017 by Registry (Talk | contribs)

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LuxCDABE (E. coli bioluminescence)

Our LIT Bulb will be composed of light sensitive E. coli that can detect sunlight (blue light) to repress bioluminescence (LuxCDABE). In the presence of light transcription of the LuxCDABE for the production of Luciferase and substrates will be inhibited. In the dark, the inhibition will be released and bioluminescence will be induced. This part encodes the necessary requirements for bioluminescence production, codon optimized for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
    Illegal AgeI site found at 2829
    Illegal AgeI site found at 3357
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 5263
    Illegal AgeI site found at 5478
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None