Part:BBa_K2332313
E-cadherin (Preproprotein) with CMV promoter & bGH poly(A) tail
| E-cadherin (Preproprotein) with CMV promoter & bGH poly(A) tail | |
|---|---|
| Function | Cell-Cell Adhesion |
| Use in | Mammalian cells |
| Abstraction Hierarchy | Device |
| RFC standard | RFC10 & RFC23 compatible |
| Backbone | pSB1C3 |
| Submitted by | [http://2017.igem.org/Team:UCL] |
This composite part is a device which efficiently encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein.
Figure 1: Construct design. The CMV promoter was fused with the E-cadherin coding part by creating a 5'-UTR (untranslated region) which is essential for efficient translation. The resulting fusion construct contains a T7 promoter according to pcDNA3 standard, a standard mammalian expression plasmid. The CMV promoter and bGH terminator were chosen to ensure efficient gene expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3212
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1414
Illegal BamHI site found at 1490
Illegal BamHI site found at 1606
Illegal BamHI site found at 2530
Illegal BamHI site found at 2832
Illegal XhoI site found at 2214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 870
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 727
Illegal BsaI site found at 1127
Illegal BsaI site found at 1534
Illegal BsaI site found at 2076
Illegal BsaI.rc site found at 968
| device_type |