Regulatory

Part:BBa_K2206000:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 20:59, 2 October 2017 by SLovat (Talk | contribs) (→‎Design Notes)


Toehold switch for hsa-miR-15b-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There must be no stop codons between the start codon and the protein coding sequence.

The RBS and start codon should not be paired to anything.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References