Regulatory

Part:BBa_K2206000:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)


Toehold switch for hsa-miR-15b-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There must be no stop codons between the start codon and the protein coding sequence.

The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References