Deleted
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Part:BBa_K2269027

Designed by: Jose Alvaro Ballesteros Gonzalez   Group: iGEM17_Valencia_UPV   (2017-09-17)
Revision as of 21:19, 17 September 2017 by Albagoutiel (Talk | contribs)


Tnos:YFP:Assembly Reporter PB:Luc:Tnos

Coding sequence corresponding to Flowering Locus T from Arabidopsis thaliana under the control of a chimeric promoter containing Etr8 (an E-responsive operator motif) fused to the minimal CMV promoter (Müller et al., 2014). FT has been optimized for Nicotiana benthamiana in order to promote floral induction in response to day length (Andres et al., 2015).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 819
    Illegal BglII site found at 302
    Illegal BglII site found at 542
    Illegal BglII site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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