Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K2269027

Designed by: Jose Alvaro Ballesteros Gonzalez   Group: iGEM17_Valencia_UPV   (2017-09-17)


Tnos:YFP:Assembly Reporter PB:Luc:Tnos

Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 819
    Illegal BglII site found at 302
    Illegal BglII site found at 542
    Illegal BglII site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 819
    Illegal XbaI site found at 506
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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