DNA
TaC1-6bp

Part:BBa_K2483005:Design

Designed by: Sophia Borowski   Group: iGEM17_Potsdam   (2017-08-30)
Revision as of 14:10, 30 August 2017 by Registry (Talk | contribs)

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sgRNA target site couples facing each other with 6 bp spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We put random big spacers (118 bp) inbetween the sgRNA target sites to reduce the amount of homologous recombination caused by the repeats.


Source

The sequence is purely synthetic.

References