Part:BBa_K2483005:Design

sgRNA target site couples facing each other with 6 bp spacer

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 562
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

To guarantee that both sgRNAs face each other, we had to make one sgRNA bind to the opposite strand. We also have big spacer of 118 bp inbetween the target ste couples to reduce homologous recombination. The recoginition sequences were analyzed with an online tool for efficacy.

Source

The sequence is purely synthetic.