Regulatory

Part:BBa_M50048:Design

Designed by: Eleanor Glockner, Jack Andraka   Group: Stanford BIOE44 - S11   (2016-12-10)
Revision as of 08:26, 12 December 2016 by Emrglock (Talk | contribs) (→‎References)


Atrazine riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replaced the aptamer in the original riboswitch with an aptamer that would target our molecule of interest, atrazine.

Source

Riboswitch components: https://parts.igem.org/Part:BBa_K784005

Atrazine aptamer: Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332

References

Wang L, Chen W, Xu D, Shim BS, Zhu Y, Sun F, Liu L, Peng C, Jin Z, Xu C, Kotov N. Nano Lett. 2009, 9(12), 4147-4152
Song, Y., Zhang, D., Polizzi, K., Zhang, E., Li, G., & Huang, W. (2012). Bacterial Whole Cell Bioreporters in Environmental Health. Biosensors and Environmental Health, 127-148. doi:10.1201/b12775-9
Park, M., Tsai, S., & Chen, W. (2013). Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery. Sensors, 13(5), 5777-5795. doi:10.3390/s130505777
Rai, N., Ferreiro, A., Neckelmann, A., Soon, A., Yao, A., Siegel, J., . . . Tagkopoulos, I. (2015). RiboTALE: A modular, inducible system for accurate gene expression control. Sci. Rep. Scientific Reports, 5, 10658. doi:10.1038/srep10658