Signalling

Part:BBa_M50027:Design

Designed by: Anthony Agbay, Tyler Dao, Vivek Lam   Group: Stanford BIOE44 - S11   (2016-11-28)
Revision as of 07:40, 12 December 2016 by Aja2488 (Talk | contribs) (→‎Source)


LuxS Synthase for Autoinducer-2 Pathway in E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 155
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required.

Source

The sequence was sourced from Uniprot under the name P45578

References

1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.