Signalling

Part:BBa_M50027:Design

Designed by: Anthony Agbay, Tyler Dao, Vivek Lam   Group: Stanford BIOE44 - S11   (2016-11-28)


LuxS Synthase for Autoinducer-2 Pathway in E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 155
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1).

Source

The sequence was sourced from Uniprot under the name P45578

References

1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.