Composite

Part:BBa_M50013

Designed by: Maddie Hayes-Lattin   Group: Stanford BIOE44 - S11   (2016-10-27)
Revision as of 02:58, 10 December 2016 by Maddiehl (Talk | contribs)

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Arsenic Sensor (arsR operon promoter and arsR gene)

This part is a subset of the ars operon, which consists of a promoter and 5 genes: arsR, arsD, arsA, arsB, and arsC. arsR is a protein that represses the transcription of the ars operon; when bound to arsenic the protein arsR no longer represses the operon and operon is transcribed. The arsD, arsA, arsB, and arsC encode proteins that give E. Coli arsenic resistance. This part consists of only the promoter and the arsR protein-encoding region to be used as an arsenic sensor. The part can be placed before any protein coding gene(s). ArsR binds to the promoter and represses transcription of the genes in the operon. When arsenic is present, it binds to the arsR repressor, and transcription of the operon occurs. Therefore, the genes in the operon will only be expressed in the presence of arsenic. For example, to test this part, we placed the arsR promoter and gene before the GFP gene and observed GFP production in the presence of arsenic.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None