User contributions
- 03:41, 10 December 2016 (diff | hist) . . (+132) . . Part:BBa M50012:Design (→Design Notes) (current)
- 03:40, 10 December 2016 (diff | hist) . . (+31) . . Part:BBa M50012 (current)
- 03:03, 10 December 2016 (diff | hist) . . (+57) . . Part:BBa M50010:Experience (→Applications of BBa_M50010) (current)
- 03:02, 10 December 2016 (diff | hist) . . (+58) . . Part:BBa M50012:Experience (→Applications of BBa_M50012) (current)
- 03:00, 10 December 2016 (diff | hist) . . (+196) . . Part:BBa M50010:Design (→Design Notes) (current)
- 02:58, 10 December 2016 (diff | hist) . . (+469) . . Part:BBa M50013 (current)
- 02:49, 10 December 2016 (diff | hist) . . (+1) . . Part:BBa M50013:Experience (→Applications of BBa_M50013) (current)
- 02:49, 10 December 2016 (diff | hist) . . (0) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 02:48, 10 December 2016 (diff | hist) . . (+47) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 02:46, 10 December 2016 (diff | hist) . . (+211) . . Part:BBa M50013:Design (→Design Notes) (current)
- 00:08, 7 December 2016 (diff | hist) . . (+5) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 00:08, 7 December 2016 (diff | hist) . . (+197) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 17:50, 5 December 2016 (diff | hist) . . (-36) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 17:49, 5 December 2016 (diff | hist) . . (+759) . . N File:Dose Response.png (Because we did not observe maximum GFP production for arsenate concentrations under 15 mM, we performed a dose response study with arsenate concentrations ranging from 0 mM to 30 mM. We grew cells in a total volume of 1.5mL LB in 37°C for 24 hours in...) (current)
- 17:48, 5 December 2016 (diff | hist) . . (-6) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 17:47, 5 December 2016 (diff | hist) . . (+1,452) . . N File:Time Course.png (We grew JM109 E. Coli cells that had been transformed with our plasmid in 0 to 15 mM KH2AsO4 concentrations for 12 hours to determine the time at which the cells began producing GFP. The total culture volume was 250uL with starting OD600 0.01 of cells...) (current)
- 17:45, 5 December 2016 (diff | hist) . . (0) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 17:41, 5 December 2016 (diff | hist) . . (0) . . File:Time Course Study.png (Maddiehl uploaded a new version of File:Time Course Study.png) (current)
- 17:39, 5 December 2016 (diff | hist) . . (+46) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:53, 4 December 2016 (diff | hist) . . (+726) . . Part:BBa M50013:Design (→Design Notes)
- 21:52, 4 December 2016 (diff | hist) . . (+351) . . Part:BBa M50013:Design (→Design Notes)
- 21:50, 4 December 2016 (diff | hist) . . (+6) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:48, 4 December 2016 (diff | hist) . . (+26) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:47, 4 December 2016 (diff | hist) . . (+345) . . N File:Time Course Study.png (A time course study for GFP production with this plasmid was also performed over 12 hours. These samples were grown in small volumes (250uL) in a plate reader at 37°. Because these growth conditions were not optimal, GFP was only expressed from the sa...)
- 21:46, 4 December 2016 (diff | hist) . . (0) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:46, 4 December 2016 (diff | hist) . . (+26) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:45, 4 December 2016 (diff | hist) . . (+1,376) . . N File:Plasmid Map.png (This plasmid map displays all the elements for the recombinant plasmid to be transformed into E. coli. All elements are contained in the plasmid Coli Comet Cassette-stuffer_Amp-HC (available from DNA 2.0) except for the arsR elements (colored yellow)....) (current)
- 21:36, 4 December 2016 (diff | hist) . . (+57) . . Part:BBa M50012:Design (→Design Notes)
- 21:35, 4 December 2016 (diff | hist) . . (+97) . . Part:BBa M50010:Design (→Design Notes)
- 21:31, 4 December 2016 (diff | hist) . . (0) . . File:GFP Production with Varied Arsenate Concentration.png (Maddiehl uploaded a new version of File:GFP Production with Varied Arsenate Concentration.png) (current)
- 21:30, 4 December 2016 (diff | hist) . . (+64) . . Part:BBa M50013:Experience (→Applications of BBa_M50013)
- 21:20, 4 December 2016 (diff | hist) . . (+156) . . Part:BBa M50013:Design (→Source)
- 21:19, 4 December 2016 (diff | hist) . . (+580) . . N File:GFP Production with Varied Arsenate Concentration.png (We transformed our plasmid containing the arsR promoter and arsR gene, amp resistance, and GFP into JM109 E. Coli cells. Wildtype JM109 wild type cells have been reported to have arsenic resistance up to 20 mM (Edmundson, M.C., Horsfall, L. 2015. Front...)
- 21:09, 4 December 2016 (diff | hist) . . (+156) . . Part:BBa M50012:Design (→Source)
- 21:09, 4 December 2016 (diff | hist) . . (+156) . . Part:BBa M50010:Design (→Source)
