Composite
STAR targe
Part:BBa_K1893009:Design
Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong Group: iGEM16_Imperial_College (2016-10-14)
Revision as of 23:48, 22 October 2016 by AkashB 10 (Talk | contribs) (Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220)
STAR inducible superfolder GFP (pAD1.S5+SF-GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 569
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 959
Illegal BsaI site found at 1137
This sequence was synthesised as a block.
Source
The pAD1.S5 attenuator sequence (STAR target)