Composite
STAR targe

Part:BBa_K1893009:Design

Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong   Group: iGEM16_Imperial_College   (2016-10-14)


STAR inducible superfolder GFP (pAD1.S5+SF-GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 569
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 959
    Illegal BsaI site found at 1137

Design Notes

This sequence was synthesised as a block with BioBrick Prefix on the 5' end and BioBrick Suffix on the 3' end.

Source

The pAD1.S5 attenuator sequence (STAR target)

References

Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220