Coding
osmY

Part:BBa_K892008:Experience

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-09-30)
Revision as of 19:40, 22 October 2016 by Frulhuq (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K892008

User Reviews

UNIQafe126cbeed9272b-partinfo-00000000-QINU

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iGEM Dundee 2016

This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS (BBa_B0030) and OsmY amplified with a C-terminal HA tag. The device showed secretion of OsmY under rhamnose-regulated conditions.

We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli No part name specified with partinfo tag. and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system



User Reviews

UNIQafe126cbeed9272b-partinfo-00000005-QINU UNIQafe126cbeed9272b-partinfo-00000006-QINU