Coding
osmY

Part:BBa_K892008:Experience

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-09-30)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K892008

User Reviews

UNIQ3af186bbccfff466-partinfo-00000000-QINU

•••••

iGEM Dundee 2016

This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS BBa_B0030) and OsmY amplified with a C-terminal HA tag resulting in the following composite part BBa_K1963007. The device showed secretion of OsmY under rhamnose-regulated conditions.

We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli BBa_K1963005 and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system



User Reviews

UNIQ3af186bbccfff466-partinfo-00000007-QINU UNIQ3af186bbccfff466-partinfo-00000008-QINU