Composite

Part:BBa_K2120308

Designed by: HongYu Yang   Group: iGEM16_BIT-China   (2016-10-13)
Revision as of 17:27, 22 October 2016 by Beaz (Talk | contribs)


B0015+LacIq+B0034+pBAD-AraC+plac+B0034+eYFP+B0015

<Description>
This part is made of three elements:
(1) Arabinose induced promoter pBAD(BBa_K2120305) which we reserve the parts BBa_K808000.This part contains the promoter as well as the coding sequence for the repressor gene araC which is transcribed in the opposite direction(“upstream”). By binding to L(+)-arabinose, AraC changes its conformation. This causes the protein to diffuses from the DNA thereby inducing transcription.
(2)lacIq, based on the part BBa_K1222003, is repeated sequence of lacI.
(3)RFP, as the report gene, can verify the pLac promoter's activity. We opposite the two promoters’ direction for reducing the interfluence of two promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1500


[edit]
Categories
Parameters
None