Protein_Domain

Part:BBa_K2017000

Designed by: Monica Victoria Gutierrez Salazar   Group: iGEM16_Valencia_UPV   (2016-10-03)
Revision as of 10:58, 21 October 2016 by Movigusa (Talk | contribs)


C-split Cas9 + DnaE C-intein

Half of the SpCas9 protein fused to DnaE C-intein. The active full-lenght spCas9 can be recovered by using the other half of the protein, N-split Cas9 + DnaE N-intein. Inteins are used to fuse both parts of the protein, as they interact and splice, leaving the active protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2020
    Illegal PstI site found at 409
    Illegal PstI site found at 643
    Illegal PstI site found at 1855
    Illegal PstI site found at 2159
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2020
    Illegal PstI site found at 409
    Illegal PstI site found at 643
    Illegal PstI site found at 1855
    Illegal PstI site found at 2159
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2020
    Illegal BamHI site found at 203
    Illegal XhoI site found at 1741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2020
    Illegal PstI site found at 409
    Illegal PstI site found at 643
    Illegal PstI site found at 1855
    Illegal PstI site found at 2159
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2020
    Illegal PstI site found at 409
    Illegal PstI site found at 643
    Illegal PstI site found at 1855
    Illegal PstI site found at 2159
    Illegal NgoMIV site found at 937
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
//chassis/eukaryote/nbenthamiana
//function/crispr
Parameters
None