Composite

Part:BBa_K1897027

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-11)
Revision as of 15:00, 18 October 2016 by Phuongnguyen (Talk | contribs)


J23100-33-ALR-Terminator

The construct contains a high expression promoter J23100 in a cassette (Part:BBa_K1897035) with a weaker RBS containing a Mixed RE Site (Part:BBa_K1897036), followed by a coding sequence of Alanine Racemase (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
    Illegal NheI site found at 854
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
    Illegal BamHI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 896
    Illegal AgeI site found at 1196
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


[edit]
Categories
Parameters
None