Composite
Part:BBa_K1897027
Designed by: Keshiniy Madivannan Group: iGEM16_NUS_Singapore (2016-10-11)
Revision as of 15:00, 18 October 2016 by Phuongnguyen (Talk | contribs)
J23100-33-ALR-Terminator
The construct contains a high expression promoter J23100 in a cassette (Part:BBa_K1897035) with a weaker RBS containing a Mixed RE Site (Part:BBa_K1897036), followed by a coding sequence of Alanine Racemase (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493
Illegal NheI site found at 854 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 778
Illegal BamHI site found at 1480 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal AgeI site found at 896
Illegal AgeI site found at 1196 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 653
[edit]
Categories
Parameters
None |