Regulatory

Part:BBa_K1897035

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-13)


High constitutive expression cassette without RBS

This derives from the high constitutive protein expression insert includes f1 origin K314110 and a high expression promoter J23100 (Part:BBa_K314100)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None