Measurement

Part:BBa_K2066037:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-08)
Revision as of 16:58, 16 October 2016 by Jlmaniaci (Talk | contribs) (→‎Source)


pACT-Tet on UNS Standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to be completed

Source

pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper,

UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"

References