Measurement
Part:BBa_K2066037:Design
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-08)
pACT-Tet on UNS Standard
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 969
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2343
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
placed on UNS 2/3 backbone for easy PCR and gibson assembly with universal primers.
Source
pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper,
UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"