Translational_Unit

Part:BBa_K2042012:Design

Designed by: Delphine Dessaux   Group: iGEM16_Evry   (2016-09-03)
Revision as of 21:50, 15 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)


RBS + PhaC_sp. MBEL 6-19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.

Source

Synthetic synthesis

References