Translational_Unit

Part:BBa_K2042012:Design

Designed by: Delphine Dessaux   Group: iGEM16_Evry   (2016-09-03)


RBS + PhaC_sp. MBEL 6-19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.

Source

The RBS were synthesized from the genomic sequence of P.putida and the PhaC gene from Pseudomonas sp. MBEL 6-19.

References