Protein_Domain
mSA2

Part:BBa_K1896000:Design

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-12)
Revision as of 17:26, 13 October 2016 by Bvh (Talk | contribs) (→‎References)


Monomeric Streptavidin (mSA2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to do


Source

Combination of streptavidin (Streptomyces avidinii) and rhizavidin (Rhizobium etli), engineered through homology modeling [1].

References

  1. Lim, K. H., Huang, H., Pralle, A., & Park, S. (2013). Stable, high‐affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnology and bioengineering, 110(1), 57-67.