Reporter

Part:BBa_K339007:Experience

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-25)
Revision as of 11:35, 10 October 2016 by Dhr9767 (Talk | contribs) (→‎Applications of BBa_K339007)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K339007

We are Team Tianjin of iGEM 2016, we aim at biodegrading PET by enzymes and we want to introduce the inclusion body regulation system in our project. This part can be used to report the generation of inclusion body in E.coli, and what we did is that we change the mRFP gene to the ddpX gene, which can cause cell lysis when overexpress. Then the E.coli will break down after too many inclusion bodies are in the periplasm so that we can directly purify the protein without the complicated step of breaking cells.

User Reviews

UNIQ35e243be8e175a57-partinfo-00000000-QINU UNIQ35e243be8e175a57-partinfo-00000001-QINU