Part:BBa_K339007:Experience

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Applications of BBa_K339007

We are Team Tianjin of iGEM 2016, we aim at biodegrading PET by enzymes and we want to introduce the inclusion body regulation system in our project. This part can be used to report the generation of inclusion body in E.coli, and what we did is that we change the mRFP gene to the ddpX gene, which can cause cell lysis when overexpress. Then the E.coli will break down after too many inclusion bodies are in the periplasm so that we can directly purify the protein without the complicated step of breaking cells.

User Reviews

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